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Anti-Cdc42 and -Rac antibodies were from Millipore. Here we describe a microporous filter method that facilitates selective purification of neurites undergoing growth or retraction. Before evaluating the relative abundance of a specific phosphopeptide in growing or retracting neurites, we first filtered the phosphopeptide dataset to remove redundancy because some peptides from the same protein may contain exactly the same phosphorylation sites due to miscleavage during tryptic digestion. Introduction Neuritogenesis is a dynamic process involving the extension of long, thin protrusions called neurites that will subsequently differentiate into long axons or an elaborate dendritic arbor 1 — 4. Epub Apr Ingenuity Pathways Analysis is a system-wide data base of biological pathways created from multiple relationships of proteins, genes, and diseases. The optimal time to harvest extending neurites for biochemical analyses was 18—20 h post-plating on the laminin-coated filters.
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Posted Fri, Sep 07 Alkane-1,2-diol-based glycosides and fatty glycosides and wax esters in Roseiflexus castenholzii and hot spring microbial mats. J Biol Chem , — Salmonella pathogenicity island 2 is expressed prior to penetrating the intestine.
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